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EMSA EMSAs were performed by mixing increasing amounts of wt or mutated BRD4 ET (with or without ASXL1 peptide at a 1:1 molar ratio) with 10 nM/lane of 147 bp 601 DNA in 2025 mM Tris pH 7.5, 150 mM NaCl and 5 mM DTT in a 10 L reaction volume

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[DOI] [PMC free article] [PubMed] [Google Scholar] 2.Llovet JM, Zucman-Rossi J, Pikarsky E, Sangro B, Schwartz M, Sherman M, Gores G