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Description

The monolayer cells were subsequently lysed with 200 L 0.1% Triton X-100 for 20 min at room temperature, and were then diluted with PBS and plated on TSB-agar plates to determine the number of intracellular bacteria (Colony-Forming Units, CFU)

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Co-authored by Angela Rice and Aimee Payne, MFA This article was co-authored by Angela Rice and by wikiHow staff writer, Aimee Payne, MFA

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