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In such studies, the extent of lung inflammation can be assessed by BALF analysis (quantification of inflammatory cells, such as neutrophils and macrophages, and molecular markers, such as cytokines and chemokines), histopathology (assessment of lung infiltration by inflammatory cells, such as neutrophils and pigmented macrophages), and both lung gene and protein expression analysis [41]

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As part of the landmark tobacco regulation bill President Obama signed in 2009, Congress required cigarette makers to set aside half the space on each of their packages for health warnings

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