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The bottom layer (chloroform phase) was collected for lipidomics analysis using LC-MS on an Orbitrap Exactive (Thermo Scientific) in line with an Ultimate 3000 LC (Thermo Scientific)
For instance, a vaper using a 12 mg/ml e-liquid in a pod system reported that it took around 10 puffs to satisfy their cravings in a way that was equivalent to smoking one cigarette
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