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Afterward, the obtained sperm pellets were treated with somatic lysis buffer (SLB: 0.1% SDS, 0.5% Triton-X-100 in DEPC water) on ice for 20 min and then centrifuged at 1000 g for 5 min

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This large population-based cohort study is the first study to use the life expectancy to assess the absolute risk from the different levels of alcohol consumption